A second task seeks to decipher the physiological role of the newly identified lamp2a homolog in fish. The growing number of fish species whose genome was completely sequenced as well as our advances in transcriptome analysis allows including in our study a large number of fish species and thus to provide a comprehensive picture of the “genetic structure” of CMA in fish. A first task aims to characterize the repertoire and the expression of genes involved in CMA in a large number of fish species. To address this issue, our strategy is based on two complementary approaches. In this project, we propose to define for the first time whether or not a CMA process is ancestral to mammalian/bird species and determine the physiological relevance of the newly identified lamp2a homolog in fish. General objective of the project and the main issues raised However, we recently identified in several fish species contigs displaying high homology with the mammalian lamp2a, suggesting that CMA appeared much earlier during evolution than initially thought. To date, CMA is presumed to be restricted to mammals and birds, due the absence of an identifiable LAMP2A, a limiting and essential protein for CMA, in phylogenetically earlier species. DS05 - Sécurité alimentaire et défi démographique LOOKING AT THE CHAPERONE-MEDIATED AUTOPHAGY IN FISH – Fish-and-Chap Looking at the chaperone-mediated autophagy in fishĬhaperone-Mediated Autophagy (CMA) is a selective mechanism for the degradation of soluble proteins in lysosomes.
0 kommentar(er)
